Dr. Mary B. Farone

Professor

Dr. Mary B. Farone
615-904-8341
Room 2085, Science Building (SCI)
MTSU Box 60, Murfreesboro, TN 37132

Degree Information

  • Ph.D., Miami University, Oxford, OH (1993)
  • M.S., Miami University, Oxford, OH (1989)
  • B.A., Thomas More College, KY (1986)

Areas of Expertise

  • Biotechnology
  • Clinical Microbiology
  • Antimicrobial agents

Biography

I am interested in the interaction of intracellular pathogens with host cells. I am particularly interested in the organisms known as "amoeba-resistant bacteria." Many of these bacteria are unculturable on laboratory media and thus often go undetected. However, many infect not only amoeba but human cells as well and may be responsible for pneumonia and other infectious diseases. These bacteria exist in both environmental and anthropogenic water sources, although many have not yet been describ...

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I am interested in the interaction of intracellular pathogens with host cells. I am particularly interested in the organisms known as "amoeba-resistant bacteria." Many of these bacteria are unculturable on laboratory media and thus often go undetected. However, many infect not only amoeba but human cells as well and may be responsible for pneumonia and other infectious diseases. These bacteria exist in both environmental and anthropogenic water sources, although many have not yet been described. The goal of the laboratory is to isolate and identify novel bacteria from amoeba and to characterize their interaction with both amoeba and human host cells utilizing techniques such as DNA sequencing, real-time quantitative PCR, and cellular staining and microscopy, including brightfield, fluorescent, confocal, and transmission electron microscopy techniques.

I am also involved in collaborative projects with faculty members in other departments. My interest in bacteria responsible for infectious disease has lead to collaboration with members of the Anthropology and Agriscience departments. We are studying the aerobic decomposition of animal s and my roles involve the prevention of environmental contamination with disease-causing microorganism as well as biodegradation of euthanizing chemicals. I have also collaborated with members of Chemistry and Mathematics departments to study the importance of various amino acids in enzyme activity. My role in this project was the mutagenesis of the DNA for bases encoding specific amino acids.

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Publications

  • Berk, S.G., Gunderson, J.H., Newsome A.L., Farone, A.L., Skolasinski, W.S., Hayes, B.J., Redding, K.S., Uddin, N., William, E.L., Reid, A., Ventrice, J.A., Skimmyhorn, J. and Farone, M. B. 2006. Occurrence of infected amoebae in cooling towers environments compared with natural aquatic environments: implications for emerging diseases. Environ. Sci. Technology 40:7440-7444.
  • Farone, A. L., O'Donnell, S.M., Brooks, C. S., Young, K. M., Pierce, J., Wetzel, D., Dermody, T. S. and ...
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  • Berk, S.G., Gunderson, J.H., Newsome A.L., Farone, A.L., Skolasinski, W.S., Hayes, B.J., Redding, K.S., Uddin, N., William, E.L., Reid, A., Ventrice, J.A., Skimmyhorn, J. and Farone, M. B. 2006. Occurrence of infected amoebae in cooling towers environments compared with natural aquatic environments: implications for emerging diseases. Environ. Sci. Technology 40:7440-7444.
  • Farone, A. L., O'Donnell, S.M., Brooks, C. S., Young, K. M., Pierce, J., Wetzel, D., Dermody, T. S. and Farone, M. B. 2006. Reovirus serotype-dependent inflammatory cytokine repsonses and replication patterns in a human monocyte cell line. Viral Immunol. 19:546-557
  • Farone, A. L., Farone, M. B., McNeal, M., Ward, R. Reoviridae and Epizootic Diarrhea of Infant Mice. 2006. In: The Mouse in Biomedical Research, 2nd Edition, J.G. Fox, C. Newcomer, A. Smith, S. Barthold, F. Quimby, M. Davisson, Eds. Academic Press, NY.
  • Farone, M., and Farone, A. 2005. Detecting mold spores in school buildings: An exercise in biodiversity. Am. Biol. Teach. 67: 401-410. Pellizzaro, A., Farone, A. Farone, M., Mushi, R. Aguinaga, M., and D. Erenso.2012. Direct laser trapping for measuring the behavior of transfused erythrocytes in a sickle cell anemia patient. Biomed Optics Express. 3:2190-2199.Jackson, J., Farone, A., and M. Farone. 2011. Bacterial enteropathogens associated with diarrhea in a rural population of Haiti [ID 23426]. Res Rep Trop Med. 2:129-133.
  • Farone, A., Farone, M., Kline, P., and Z. Sinkala. 2010. A practical approach for computing the active site of the ribonucleoside hydrolase of E.coli encoded by rihC. Adv Exp Med Biol. 680:437-443.
  • Farone, A., Farone, M., Khaliq, A., Kline, P., Quinn, T., and Z. Sinkala, 2009. Identifying the active site of ribonucleoside hydrolase of E. coli encoded by RihC. Proceedings of the 2009 International Conference on Bioinformatics & Computational Biology: BIOCOMP2009 Vol. I, Editors: H.R. Arabnia and M. Q. Yang, CSREA Press, USA, pp 216-218.
  • Pellizzaro, A., Farone, A., Farone, M., Mushi, R., Aguinaga, M., and D. Erenso. 2012. Direct laser trapping for measuring the behavior of transfused erythrocytes in a sickle cell anemia patient. Biomed Optics Express. 3:2190-2199.
  • Jackson, J., Farone, A., and M. Farone. 2011. Bacterial enteropathogens associated with diarrhea in a rural population of Haiti [ID 23426]. Res Rep Trop Med. 2:129-133.
  • Farone, A., Farone, M., Kline, P., and Z. Sinkala. 2010. A practical approach for computing the active site of the ribonucleoside hydrolase of E.coli encoded by rihC. Adv Exp Med Biol. 680:437-443.
  • Farone, A., Farone, M., Khaliq, A., Kline, P., Quinn, T., and Z. Sinkala, 2009. Identifying the active site of ribonucleoside hydrolase of E. coli encoded by RihC. Proceedings of the 2009 International Conference on Bioinformatics & Computational Biology: BIOCOMP2009 Vol. 1, Editors: H.R. Arabnia and M. Q. Yang, CSREA Press, USA, pp 216-218.
  • Ward, R.L., McNeal, M.M., Farone, M.B., and A.L. Farone. 2007. "The Reoviridae" In: The Mouse in Biomedical Research. (A.Smith, ed.) 2nd Edition. Elsevier Scientific Publishing, Inc., New York. pp. 235-268.

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